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KMID : 0895820140240030011
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Journal of Oriental Rehabilitation Medicine
2014 Volume.24 No. 3 p.11 ~ p.27
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Effect of Mixed Extract of Ephedrae Sinica and Fibrosum Gypsum on Differentiation of Preadipocytes and Obesity of Rats
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Ki Young-Bum
Kim Dae-Hun
Yang Mi-Sung
Kang Dae-Hee
Kim Sun-Jong
Choi Jin-Bong
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Abstract
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Objectives : This study was designed to evaluate the effects of Ephedra Sinica and Fibrosum Gypsum extract on obesity by using 3T3-L1 cells and high fat diet rats.
Methods : In vitro, Ephedra Sinica and Fibrosum Gypsum extract (50, 100, 200, 500 μg/ ml) were added in 3T3-L1 cells. Cytotoxicity was measured by MTT assasy. Adipocyte differentiation was measured by Oil Red O staining, GPDH activity and C/EBPα protein expression. In vivo, Sprague-Dawley rats were divided into 5 groups : Normal diet group (Normal group), taken high fat diet and no treatment group (Control group), taken high fat diet and orally administered Ephedra Sinica and Fibrosum Gypsum extract daily (Group I: 50 mg/kg, Group II: 100 mg/kg, Group III : 200 mg/kg, oral). For 6 weeks of administration, body weight and the amount of food intake were measured once a week. After administration, blood analysis (AST, ALT, T-Bilirubin, BUN, RBC, Hb, HCT), serum lipid level (triglyceride, Total cholesterol, HDL, LDL), serum leptin level, epididymal adipose tissue weight and histological finding of liver were estimated.
Results : In vitro, The cytotoxicity was not significant. 3T3-L1 cell’s differentiation was significantly decreased in Oil Red O staining, GPDH activity and C/EBPα protein expression. In vivo, Body weight and the amount of food intake, AST, ALT, Total cholesterol, TG, LDL, serum leptin, epididymal adipose tissue weight showed significant decrease in group I, group II and group III. There were no significant difference in T-bilirubin, BUN, RBC, Hb and HCT between all groups. HDL showed significant increase in group I, group II and group III. In histological finding of liver tissue, there were decreased adiposity and cytopathic effect in group I, group II and group III.
Conclusions : It is suggested that Ephedra Sinica and Fibrosum Gypsum extract can be used in the treatment of obesity.
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KEYWORD
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Obesity, 3T3-L1 cell, Rats fed high fat diet, Ephedra Sinica, Fibrosum Gypsum
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